Detection of herpes simplex virus type 2-specific antibody with glycoprotein G. Lee FK, Coleman RM, Pereira L, Bailey PD, Tatsuno M, Nahmias AJ.
#UNIVERSITY OF WASHINGTON HSV WESTERN BLOT TRIAL#
A trial of topical acyclovir in genital herpes simplex virus infections. Corey L, Nahmias AJ, Guinan ME, Benedetti JK, Critchlow CW, Holmes KK.Presence of type 2 antibody in acute serum samples. Serologic analysis of first-episode nonprimary genital herpes simplex virus infection. Humoral immune response to HSV-1 and HSV-2 viral proteins in patients with primary genital herpes. Use of densitometric analysis for interpreting HSV serologies based on Western blot. Detection of type-specific antibody to herpes simplex virus type 1 by radioimmunoassay with herpes simplex virus type 1 glycoprotein C purified with monoclonal antibody. Arvin AM, Koropchak CM, Yeager AS, Pereira L.Links to PubMed are also available for Selected References. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page.
#UNIVERSITY OF WASHINGTON HSV WESTERN BLOT FULL#
Full textįull text is available as a scanned copy of the original print version. WBA was more sensitive than IEA in detecting seroconversion following primary HSV-1 genital herpes, although both assays may miss persons undergoing early seroconversion to HSV-2. Both WBA and IEA are accurate and sensitive tests for HSV-2 antibody in patients convalescing from a first episode or having symptomatic or asymptomatic recurrent genital herpes. In patients with culture-proven primary genital HSV-1 infection, WBA detected antibodies to HSV-1 proteins in 16 of 17 (94%) serum samples drawn at least 21 days after onset of primary genital HSV-1 infection, compared with 9 of 17 (53%) serum samples tested for gG-1 by IEA. Of sera drawn earlier than 21 days from onset of primary genital HSV-2, antibodies to HSV-2 were detected in 25% by WBA and 8% by IEA. Both tests were most sensitive with sera obtained at least 21 days after onset of primary HSV-2 infections or sera drawn during recurrent HSV-2 genital episodes: 75 of 76 (99%) such serum samples were positive for HSV-2 antibody by WBA and 73 of 76 (96%) were positive by IEA. Of 137 serum samples tested, none was mistyped by either WBA or IEA. Sera from patients with culture-proven genital herpes infections were tested for herpes simplex virus type 1 (HSV-1)- and HSV-2-specific antibodies by both a Western blot (immunoblot) technique (WBA) and immunodot enzyme assays (IEAs) specific for HSV-1 or HSV-2 glycoprotein G (gG).
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